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Trypsin Like Enzyme ELISA Kit
Item number：KET01-96/KET02-96
Specs：96T
Sell-by date：12 months
Storage conditions：2-8℃
Technical support：19503412257
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Trypsin Like Enzyme ELISA Kit


Product Introduction

In December 2022, the National Institutes for Food and Drug Control published a research paper titled "Establishment and Validation of a Double-antibody Sandwich Quantitative ELISA for TrypLE" in Chinese Journal of Biologicals. Junyan Biotech participated in the publication of this article. In this study, orthogonal experiments were used to determine the optimal concentrations of the capture antibody and detection antibody. A double - antibody sandwich quantitative ELISA method for TrypLE was established and verified for its linear range, specificity, limit of detection, limit of quantification, accuracy, and repeatability. The applicability of the method was verified by detecting various biological products using the established method.


After strict methodological verification, Junyan Biotech independently developed an ELISA - based recombinant trypsin - like analogue quantitative detection kit, which can accurately identify multiple imported and domestic recombinant trypsins and their analogues, including TrypLE™ from Gibco.


The Recombinant Trypsin - like Analogue Quantitative Detection Kit (Catalog No. KET01 - 96) can quickly and effectively detect the residual amount of TrypLE™ in various samples. For stem cell users, Junyan Biotech has launched a second - generation product (Catalog No. KET02 - 96), which has lower sensitivity (the limit of quantification is as low as 0.1 ng/mL) to better meet diverse detection needs.

Advantages and Features

  • Market First: The first product on the market for TrypLE™ residual detection.

  • Efficient Operation: The whole process takes a short time, within 2 hours in total.

  • Simple Operation: All components are ready - to - use. There is no need to prepare standard products, and the operation is convenient and simple.

  • Superior Performance: Strong specificity, high accuracy, and good repeatability.

  • Wide Applicability: Can detect multiple recombinant trypsin analogues.

Performance Indicators

KET01 - 96KET02 - 96
Detection System30 + 30+15 min60 + 60 + 15 min
Detection Range0.41 - 40 ng/mL0.1 - 16 ng/mL
Limit of Detection≤ 0.4 ng/mL≤ 0.1 ng/mL
Limit of Quantification0.5 ng/mL0.1 ng/mL
Spike Recovery Rate80% - 120%80% - 120%
Repeatability (Intra - batch CV)≤ 10%≤ 10%

Data Results

  • Standard Curve Validation:
    The standard curves are obtained by 4 - PL fitting. The detection range of KET01 - 96 is 0.41 - 40 ng/mL, and that of KET02 - 96 is 0.1 - 16 ng/mL. The R² values of both can reach 0.99.

  • Kit Versatility:
    Junyan Biotech's Recombinant Trypsin - like Analogue Quantitative Detection Kit can detect multiple imported and domestic recombinant trypsins and their analogues, including TrypLE™ from Gibco.

    • A. The kit can stably detect the concentrations of recombinant trypsins and their analogues from different manufacturers.

    • B. The dilution linearity of recombinant trypsins and their analogues from different manufacturers detected by the kit is greater than 0.99.

Detection Principle

This kit uses the double - antibody sandwich enzyme - linked immunosorbent assay (ELISA) technology to determine the trace residual amount of TrypLE™ in samples. The 96 - well microplate is coated with a capture antibody to form a solid - phase antibody. Then, standard products and test samples are added, followed by a detection antibody labeled with horseradish peroxidase (HRP). A sandwich complex of solid - phase antibody - TrypLE™ - labeled antibody is formed. After the reaction, the plate is washed, and then a substrate is added for a color - development reaction. The substrate is converted into blue under the catalysis of HRP and then into yellow under the action of a stop solution (as shown in the figure below). The absorbance (OD value) is measured at a wavelength of 450 nm, and the content of TrypLE in the test sample is calculated through the standard curve.


Note: TrypLE™ is a registered trademark of Thermo Fisher Scientific.


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