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Collagenase Type I ELISA Kit
Item number：CI0111B-96
Specs：96T
Sell-by date：12 months
Storage conditions：2-8℃
Technical support：19503412257
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Collagenase Type I ELISA Kit

Product Introduction

Collagenase is used to dissociate various tissues into single cells in vitro, making it suitable for single cell isolation of organs and tissues in various research applications. Regulations governing biological product production emphasize the necessity of testing cell culture-related materials, thereby increasing the importance of detecting collagenase residues.

Junyan Bioscitech’s "Collagenase Type I Quantitative Detection Kit" is developed using a polyclonal antibody-based double-antibody sandwich ELISA. Specifically designed for collagenase mixtures derived from Clostridium histolyticum, this kit enables rapid and efficient detection of collagenase residues in various samples.

Advantages and Features

  • Accurate Detection:

    Polyclonal antibodies prepared against collagenase mixtures ensure effective quantification.

  • High Specificity:

    No cross-reactivity with other non-target proteins commonly used in cell culture.

  • Superior Performance:

    • Low limit of quantitation (LOQ): 0.625 ng/mL.
    • High accuracy and excellent repeatability.

  • Broad Applicability:

    Users can establish standard curves using specific sources of collagenase employed in their production processes, further ensuring result accuracy.

Performance Indicators

  • Reaction System: 60 + 60 + 20 minutes

  • Spike Recovery Rate: 80%–120%

  • Limit of Quantitation (LOQ): 0.625 ng/mL

  • Repeatability (Intra-Batch CV): ≤10%

  • Detection Range: 0.625–20 ng/mL

  • Measurement Deviation: ≤15%



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