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Collagenase Type I ELISA Kit
Item number：CI0111B-96
Specs：96T
Sell-by date：12 months
Storage conditions：2-8℃
Technical support：19503412257
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Collagenase Type I ELISA Kit

Product Introduction

Collagenase is used to dissociate various tissues into single cells in vitro, making it suitable for  single-cell isolation of organs and tissues in various research applications. Regulations governing biological product production emphasize the necessity of testing cell culture-related materials, thereby increasing the importance of detecting collagenase residues.

Junyan Biotech’s "Collagenase Type I Quantitative Detection Kit" is developed using a polyclonal antibody-based double-antibody sandwich enzyme-linked immunosorbent assay (ELISA). Specifically designed for collagenase mixtures derived from Clostridium histolyticum, this kit enables rapid and efficient detection of collagenase residues in various samples.

Advantages and Features

  • Accurate Detection: Polyclonal antibodies prepared against collagenase mixtures ensure effective quantification.

  • High Specificity: No cross-reactivity with other non-target proteins commonly used in cell culture.

  • Superior Performance:

    • Low limit of quantitation (LOQ): 0.625 ng/mL.
    • High accuracy and excellent repeatability.

  • Broad Applicability: Users can establish standard curves using specific sources of collagenase employed in their production processes, further ensuring result accuracy.

Performance Indicators

  • Reaction System: 60 + 60 + 20 minutes

  • Spike Recovery Rate: 80%–120%

  • Limit of Quantitation (LOQ): 0.625 ng/mL

  • Repeatability (Intra-Batch CV): ≤10%

  • Detection Range: 0.625–20 ng/mL

  • Measurement Deviation: ≤15%



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