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CHO HCP ELISA Kit
Item number：HCH0111A-96
Specs：96T
Sell-by date：12 months
Storage conditions：2-8℃
Technical support：19503412257
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CHO HCP Residual Detection Kit

Product Introduction


Chinese Hamster Ovary (CHO) cells are one of the most successful cell lines for expressing heterologous proteins and are widely used in bioengineering. As foreign proteins in the human body, CHO cell-derived host cell proteins (HCPs) may induce immune responses when their levels reach 100 ppm in vivo. Therefore, controlling HCP residues is critical during biological product manufacturing. Detection of CHO cell HCP residues is one of the important indicators for evaluating bioprocess quality.
Junyan Biotech’s independently developed CHO HCP Residual Detection Kit (Enzyme-Linked Immunosorbent Assay) is a highly sensitive, universal assay for detecting CHO HCP residues in biological products at various process stages. The kit enables sensitive, specific, and accurate quantification of CHO cell host protein residuals.


Advantages and Features


  • Comprehensive Antibody Coverage: Broad antibody panel ensures compatibility throughout the product lifecycle.

  • High Specificity: No cross-reactivity with BSA, Protein A, HEK293 HCP, or E. coli HCP.

  • Superior Performance: Excellent standard curve linearity, high accuracy, good repeatability, and qualified sample spike recovery rates.


Performance Indicators


  • Reaction System: 2 hours + 0.5 hours

  • Limit of Quantitation (LOQ): 3 ng/mL

  • Detection Range: 3–486 ng/mL

  • Spike Recovery Rate: 80%–120%

  • Limit of Detection (LOD): ≤1 ng/mL

  • Repeatability (Intra-Batch CV): ≤15%


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