CAR/TCR Gene Copy Number (qPCR) Detection Kit-Safety Detection-君研生物科技(山西)有限公司

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CAR/TCR Gene Copy Number (qPCR) Detection Kit

Item number：CT0311A-100

Specs：100R

Sell-by date：12months

Storage conditions：-20℃

Technical support：19503412257

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CAR/TCR Gene Copy Number (qPCR) Detection Kit

Product Introduction

Independently developed by Junyan Bio, the CAR/TCR Gene Copy Number (qPCR) Detection Kit features strong specificity and high sensitivity. It adopts the multiplex fluorescent probe qPCR method, enabling rapid and specific quantitative detection of the copy numbers of CAR or TCR genes in the genome of CAR-T or TCR-T cells, as well as the single-copy gene (SCG) in human cells. Through calculation, the average copy number of the target gene per cell in the sample can be obtained.

This kit can be used in conjunction with Junyan Bio's "Host Cell Residual DNA (Magnetic Bead Method) Sample Pretreatment Kit" and automatic nucleic acid extractors.

Formula: Plasmid copy number (copies/μL) = 6.02×10¹⁴ × plasmid concentration (ng/μL) / (number of plasmid base pairs × 660)

Performance Indicators

Linear range: 3×10⁶ copies/µL - 3×10¹ copies/µL.
Standard linearity: R² ≥ 0.980, amplification efficiency 90% ≤ Eff (%) ≤ 110%, CV of detection values at each concentration < 15%.
Accuracy (spiked recovery rate): 70% - 130%.
Limit of quantitation: 10 copies/µL, CV ≤ 20%, measurement deviation not exceeding |±20%|.