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CHO Residual DNA (qPCR) Detection Kit
Item number：CH0311C-100
Specs：100 R
Sell-by date：12 months
Storage conditions：-20℃
Technical support：19503412257
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CHO Residual DNA (qPCR) Detection Kit


Product Introduction

As one of the most commonly used tool cells, CHO cells are widely applied in the biopharmaceutical industry. However, residual host cell DNA entering the human body through biological products poses uncontrollable risk factors. Therefore, regulatory authorities such as WHO, FDA, and China's NMPA have strict limits on the residual amount of host cell DNA to ensure nucleic acid residues remain within safe and controllable ranges.
Junyan Biotech’s independently developed CHO residual DNA (qPCR) detection kit features strong specificity and high sensitivity. It uses the qPCR method to quantitatively detect CHO cell DNA residues in semi-finished products, intermediate products, and finished products of biological preparations.


Advantages and Features


  • High Stability: Less affected by environmental factors, with stable performance throughout the shelf life.

  • High Precision:

    • Low-concentration repeatability CV value < 20%;
    • Medium/high-concentration repeatability CV value < 15%.

  • Strong Specificity: Unaffected by exogenous DNA from Human, E. coli, or Vero cells.

  • Rapid and Convenient: Easy to operate, with results available within 1 hour.


Performance Indicators


  • Linear Range of Standard Curve: 3 fg/μL – 300 pg/μL

  • Reference Materials: Traceable to national standard reference materials

  • Limit of Quantitation (LOQ): 3 fg/μL

  • Detection Principle: Fluorescent probe qPCR method

  • Fluorescent Group: FAM

  • Applicable Instruments: Suitable for various domestic and imported quantitative PCR instruments


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