E1A&SV40LTA Residual DNA (qPCR) Detection Kit-Host Cell Residual DNA Detection-君研生物科技(山西)有限公司

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E1A&SV40LTA Residual DNA (qPCR) Detection Kit

Item number：ESV0311C-100

Specs：100R

Sell-by date：12 months

Storage conditions：-20℃

Technical support：19503412257

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E1A&SV40LTA Residual DNA (qPCR) Detection Kit

Product Introduction

With the development of biotechnology, various viral vectors are used in the research, development, and production of gene and cell therapy products. For specific transforming sequences with known potential safety risks in these products, such as the E1A sequence and SV40 large T - antigen sequence carried by HEK293T cells, residual control should be carried out separately to reduce uncontrollable risk factors.

The E1A&SV40LTA Residual DNA (qPCR) Detection Kit independently developed by Junyan Biotech uses the multiplex fluorescent quantitative PCR method to quantitatively detect the DNA residues of E1A and SV40LTA in semi - finished products, intermediate products, and finished products. This kit is used in conjunction with Junyan Biotech's host cell residual DNA sample pretreatment kit to accurately quantify trace amounts of residual E1A and SV40LTA DNA in samples.

Advantages and Features

High Precision: The coefficient of variation (CV) for low - concentration repeatability is less than 20%, and for medium - and high - concentration repeatability is less than 15%.
Rapid and Efficient: Detection is fast, and results can be obtained within one hour.
High Sensitivity: The limit of quantification is 4 copies/μL.

Performance Indicators

Linear Range of Standard Curve: 4×10⁶～4×10¹ copies/μL
Reference Material: Linearized DNA quantitative reference material
Limit of Quantitation (LOQ): 4 copies/μL
Detection Principle: Multiplex fluorescent probe qPCR method
Fluorescent Groups: FAM, CY5
Applicable Instruments: Suitable for a variety of domestic and imported quantitative PCR instruments
Data Results: The data results will be presented in a clear and reliable way, which can be used for accurate analysis of E1A and SV40LTA DNA residues.