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PG13 Residual DNA (qPCR) Detection Kit
Item number:PG0311A-100
Specs:100 R
Sell-by date:12 months
Storage conditions:-20℃
Technical support:19503412257
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PG13 Residual DNA (qPCR) Detection Kit


Product Introduction

The PG13 cell line is derived from mouse embryonic fibroblasts (N1H3T3) modified by integrating the Moloney murine leukemia virus (MLV) expression vector and the gibbon ape leukemia virus (GALV) envelope protein gene. As a packaging cell line, PG13 is primarily used in the development of cell products (such as CAR-T/TCR-T cells). It is typically employed to package retroviruses, produce retroviral vectors, and infect patient T lymphocytes to generate cell preparations for gene and cell therapy.


This kit, equipped with PG13 DNA quantitative reference materials, enables rapid and accurate quantification of PG13 residual DNA in intermediate, semi-finished, and finished biological products. The detection range of the kit is 300 pg/μL to 0.003 pg/μL. It can be used in conjunction with Junyan Biotech’s host cell residual DNA sample pretreatment kit and fully automated nucleic acid extractors.

Advantages and Features

  • High Stability: Less affected by environmental factors, with stable performance throughout the shelf life.

  • High Precision: Coefficient of variation (CV) of detection values at all concentrations < 15%.

  • Strong Specificity: Interference tests with commonly used DNA in biopharmaceutical production show that the interference group overlaps with the control group, indicating no interference.

  • Rapid and Convenient: Easy to operate, with results available within 1 hour.

Performance Indicators

  • Linear Range of Standard Curve: 3 fg/μL – 300 pg/μL

  • Negative Reference Material Compliance Rate: NTC (No Template Control) shows "Undetermined" or Ct value > 35.

  • Limit of Quantitation (LOQ): 3 fg/μL

  • Detection Principle: Fluorescent probe qPCR method

  • Fluorescent Group: FAM

  • Applicable Instruments: Suitable for various domestic and imported quantitative PCR instruments

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