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Hela Residual DNA (qPCR) Detection Kit
Item number:H0311C-100
Specs:100 R
Sell-by date:12 months
Storage conditions:-20℃
Technical support:19503412257
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Hela Cell Residual DNA (qPCR) Detection Kit


Product Introduction

Hela cells are widely used in protein expression and rAAV production, and their residual DNA may exist as impurities in semi-finished products, intermediate products, and finished products. Due to the stability of DNA, residues are prone to accumulate during production. When these biological products are applied to human disease treatment, they may pose uncontrollable risk factors. Therefore, the detection of nucleic acid residues is a mandatory item in all quality control standards, requiring compliance with regulatory guidelines of WHO, FDA, and China's NMPA to ensure residues are within safe and controllable limits.


The Hela cell residual DNA (qPCR) detection kit independently developed by Junyan Biotech features high specificity, enabling rapid and specific detection of Hela cell DNA residues in semi-finished products, intermediate products, and finished products.


Advantages and Features


  • High Precision:

    • Low-concentration repeatability CV value < 20%;

    • Medium/high-concentration repeatability CV value < 15%.

  • Linear Standard:
    Developed in accordance with national basic requirements for standard curve method establishment, with comprehensive indicators, high standards, and stable/reliable results.

  • Rapid and Efficient:
    Fast detection with results available within one hour.

  • Market First:
    Currently the first kit on the market for detecting Hela cell residual DNA.


Performance Indicators


  • Linear Range of Standard Curve: 30 fg/μL – 300 pg/μL

  • Reference Materials: Accurate and reliable

  • Limit of Quantitation (LOQ): 30 fg/μL


Detection Principle
Fluorescent probe qPCR method


Fluorescent Group
FAM


Applicable Instruments
Suitable for multiple domestic and imported quantitative PCR instruments


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