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HEK293 Residual DNA (qPCR) Detection Kit
Item number:H20311D-100
Specs:100 R
Sell-by date:12 months
Storage conditions:-20℃
Technical support:19503412257
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HEK293 Residual DNA (qPCR) Detection Kit (H20311D - 100)


Product Introduction


With the rapid development of gene and cell therapy, various viral vectors are used in gene and cell therapy. Most of these vectors are produced using 293 series cells (such as HEK293, 293T, etc.), which leads to the presence of 293 series cell residual DNA as an impurity in the final vector product. Due to the stability of host cell DNA, it is prone to residue during the production process. When these biological products are applied to the treatment of human diseases, they may bring uncontrollable risk factors. Therefore, the detection of host nucleic acid residues is a mandatory item in various quality control standards. It is necessary to comply with the regulatory guidelines of WHO, FDA, and China's NMPA to ensure that host nucleic acid residues are within a safe and controllable range.
The HEK293 residual DNA detection kit independently developed by Junyan Biotech has strong specificity and can quickly and specifically detect DNA residues of all 293 series cells (such as HEK293, 293T, etc.) in biological products and gene and cell therapy products.


Advantages and Features


  • High Precision:

    • CV value of low - concentration repeatability < 20%;

    • CV value of medium - and high - concentration repeatability < 15%.

  • Linear Standard:
    In accordance with the basic requirements of national standards, it has comprehensive indicators, high standards, and stable and reliable results.

  • Rapid and Efficient:
    The detection is fast, and results can be obtained within one hour.


Performance Indicators


  • Linear Range of Standard Curve: 30 fg/μL - 300 pg/μL

  • Reference Substances: Accurate and reliable

  • Limit of Quantitation (LOQ): 30 fg/μL


Detection Principle
Fluorescent probe qPCR method


Fluorescent Group
FAM


Applicable Instruments
Suitable for various domestic and imported quantitative PCR instruments

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