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PG13 Residual DNA Fragment (qPCR) Detection Kit
Item number：PG0311A-3×100
Specs：3×100R
Sell-by date：12 months
Storage conditions：-20℃
Technical support：19503412257
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PG13 Residual DNA Fragment (qPCR) Detection Kit

Product Introduction

As a primary packaging cell line for retroviral vector production, PG13 cells may introduce cell-derived residual host DNA impurities during production and purification processes. These residual DNA fragments pose potential risks. Therefore, global pharmaceutical regulatory authorities require monitoring and control of exogenous host DNA residues in biological products to ensure their safety and quality controllability.


Junyan Biotech’s independently developed PG13 residual DNA fragment (qPCR) detection kit enables rapid and accurate quantification of PG13 residual DNA fragments in intermediate, semi-finished, and finished biological products. The kit has a linear range of 300 pg/μL to 0.003 pg/μL for fragments of  120 bp, 259 bp and 505 bp. It is used in conjunction with Junyan Biotech’s "Host Cell Residual DNA Sample Preparation Kit (Magnetic Bead)".

Advantages and Features

  • Comprehensive Fragment Coverage:
    Detects PG13 residual DNA fragments of 120 bp, 259 bp and 505 bp, covering potential functional gene sizes to meet customer needs.

  • High Sensitivity & Wide Linear Range:

    • Linear range: 300 pg/μL - 0.003 pg/μL (for all fragment sizes)

    • Sensitivity as low as 3 fg/μL, enabling precise analysis of DNA fragment distribution.

Performance Indicators

  • Linear Range: 300 pg/μL ~ 0.003 pg/μL (for 120 bp, 259 bp, 505 bp fragments)

  • Standard linearity: R² ≥ 0.980; amplification efficiency: 90% ≤ Eff (%) ≤ 110%; CV of detection values at each concentration < 15%;


  • Accuracy (spiked recovery rate): 70% - 130%;


  • Limit of quantitation (LOQ): 0.003 pg/μL; CV ≤ 20%; measurement deviation not exceeding |±20%|.
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