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Vero Residual DNA Fragment (qPCR) Detection Kit
Item number：FV0311B-4×100
Specs：4×100 R
Sell-by date：12 months
Storage conditions：-20℃
Technical support：19503412257
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Vero Residual DNA Fragment (qPCR) Detection Kit


Product Introduction

The Vero cell line is a commonly used immortalized cell line in viral vaccine production. Residual DNA from host cells poses risks of infectivity, immunogenicity, or carcinogenicity. The maximum acceptable level of residual DNA is 10 ng/dose, while the requirement for DNA fragment size is smaller than the length of a functional gene (the minimum length of a functional gene is 200 bp). Fragments larger than 200 bp carry greater potential risks, making it essential for vaccine manufacturers to test the size distribution of residual DNA fragments in finished products.
Junyan Biotech’s independently developed Vero residual DNA fragment (qPCR) detection kit is used to quantitatively analyze the size distribution of Vero host cell DNA residual fragments in intermediate, semi-finished, and finished biological products. Based on the principle of PCR fluorescent probe technology, the kit designs four different amplified fragments (86 bp, 154 bp, 242 bp, 540 bp) to rapidly and specifically detect DNA fragment distribution. It can be used in conjunction with our company’s host residual DNA sample pretreatment kit.


Advantages and Features


  • Linear Standard: Developed in compliance with national requirements for standard curve establishment, with comprehensive indicators and high standards.

  • High Precision:

    • Low-concentration repeatability CV value < 20%;
    • Medium/high-concentration repeatability CV value < 15%.

  • High Stability: Less affected by environmental factors, with stable performance within the shelf life.

  • Rapid and Efficient: Fast detection with results available within one hour.


Performance Indicators


  • Linear Range (Fragments < 200 bp): 3 fg/μL – 300 pg/μL

  • Fluorescent Group: FAM

  • Linear Range (Fragments > 200 bp): 30 fg/μL – 300 pg/μL

  • Detection Principle: Fluorescent probe qPCR method

  • Applicable Instruments: Suitable for various domestic and imported quantitative PCR instruments

  • Reference Standards: Traceable to national standard reference materials


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